Normalized Index of Hemolysis

Normalized Index of Hemolysis (NIH) is the clinical measure of hemolysis as per the ASTM standards.
NIH (g/100L) = \delta_PfHb x V x [ (100-Hct)/Hct ] x [ 100 / (Q x T) ]
where:
a.   \delta_PfHb = increase in plasma free hemoglobin concentraion (g/L)
b.   V = volume of blood in mock loop circuit (L)
c.   Hct = hematocrit
d.   Q = flow rate (L/min)
e.   T = Time (min)
NIH can be stated as:
Amount of PfHb released per pass of blood volume through the blood pump.
The hemolysis ratio which is more commonly used in the simple shear hemolysis experiments can be converted to NIH as:
NIH (g/100L) = 100 x [\delta_Hb / Hb] x [1 - Hct/100] x k where:
a.   [\delta_Hb / Hb] = hemolysis ratio
b.   Hct = hematocrit
c.   k = hemoglobin content of blood (150g/L) for healthy person